Batch cultures of Chlorella sorokiniana DOE1412 grown in raceway ponds at the PNNL testbed site in Arizona. Photo credit: Michael Huesemann, PNNL.

Micrograph showing cells of Chlorella sorokiniana DOE1412 cultivated in BG11 medium. Cells were in an early growth phase and did not induce lipid accumulation, consequently no cytosolic oil bodies are visible. Note that cells have an apparent pyrenoid. Photo credit: Juergen Polle, Brooklyn College of CUNY.

The Chlorella sorokiniana DOE1412 genome sequence and gene models have not been determined by the JGI, but were downloaded from the Los Alamos National Laboratory (LANL) Greenhouse portal on October 31, 2019. In order to ensure this genome is comparable to those sequenced by the JGI, we applied filters to remove if present: 1) transposable elements, 2) pseudogenes, 3) alternative transcripts and overlapping models, 4) alleles on secondary scaffolds and 5) unsupported short models. This resulted in the removal of 2,354 models and the generation of the FilteredModels1 (GeneCatalog) gene track. All published models are available in the ExternalModels track. Please note that this copy of the genome is not maintained by LANL and is therefore not automatically updated. In order to allow comparative analyses with other algal genomes sequenced by the JGI, a copy of this genome is incorporated into PhycoCosm. The JGI Annotation Pipeline was used to add functional annotation to this genome.

Chlorella sorokiniana DOE1412 (=UTEX B 3016) belongs to the class of the Trebouxiophyceae within the phylum of the green algae. It thrives in fresh water and is of interest for studies on lipid production. This strain of C. sorokiniana had originally been isolated as a contaminant of another green algal culture in the Juergen Polle laboratory at Brooklyn College of the City University of New York during the National Alliance for Advanced Biofuels and Bioproducts (NAABB) project and it was originally described by Neofotis et al. (2016). The strain performed well in initial studies under a variety of conditions and it became a new potential platform as a feedstock for production of biofuels (Lammers et al., 2017). Later pond infections with the predator bacterium Vampirovibrio chlorellavorus limited further outdoor cultivation of DOE1412 (Park et al., 2019). However, strain DOE1412 is now a model for studies of algal host-pathogen interactions. Note that the strain can be obtained from the UTEX culture collection.