Aliquandostipite khaoyaiensis is a member of Aliquandostipitaceae family from the order Jahnulales (Dothideomycetes), and was sequenced as part of the 1000 Fungal Genomes Project. Dothideomycetes is the largest and most diverse class of ascomycete fungi. It comprises 11 orders 90 families, 1300 genera and over 19,000 known species. The 1000 Fungal Genomes Project aims to fill in gaps in the Fungal Tree of Life by sequencing at least two reference genomes from the more than 500 recognized families of Fungi. This project additionally aims to inform research on plant-microbe interactions, microbial emission and capture of greenhouse gasses, and environmental metagenomic sequencing.