Understanding how cellular machinery can be altered to promote enhanced expression and secretion of native enzymes is of great importance to biotechnology. On a broader perspective, characterization of fungal secretion mechanisms is also for scientific fields, such as investigations into fungal pathogenicity. In industry and in the lab, enhanced production of enzymes is often achieved through mutagenesis and selection for enzyme hyperproducer mutants. One such Aspergillus lineage, Parent ATCC 13496; Mutant ATCC 13497, has been generated for the hyperproduction of an important enzyme glucoamylase. Sequencing of each strain in the lineage will enable the identification of the genomic changes that endow the mutants with the hyperproduction phenotype. Identification of these gemomic lesions will help elucidate the mechanisms behind enzyme hyperproduction.
Genome Reference(s)
Vesth TC, Nybo JL, Theobald S, Frisvad JC, Larsen TO, Nielsen KF, Hoof JB, Brandl J, Salamov A, Riley R, Gladden JM, Phatale P, Nielsen MT, Lyhne EK, Kogle ME, Strasser K, McDonnell E, Barry K, Clum A, Chen C, LaButti K, Haridas S, Nolan M, Sandor L, Kuo A, Lipzen A, Hainaut M, Drula E, Tsang A, Magnuson JK, Henrissat B, Wiebenga A, Simmons BA, Mäkelä MR, de Vries RP, Grigoriev IV, Mortensen UH, Baker SE, Andersen MR
Investigation of inter- and intraspecies variation through genome sequencing of Aspergillus section Nigri.
Nat Genet. 2018 Dec;50(12):1688-1695. doi: 10.1038/s41588-018-0246-1
