Info • Basidiobolus microsporus NRRL 2992 v1.0


[September 2022] The Basidiobolus microsporus NRRL 2992 v1.0 genome was sequenced with PacBio, assembled with Flye, and annotated with the JGI Annotation Pipeline. Mitochondrial genome was assembled separately and is available in the downloads section.

This genome is likely a polymorphic diploid/dikaryon, and this is reflected in an assembly and annotation with significant separation of alleles. Many of the scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertiore of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.

Genome Assembly
Genome Assembly size (Mbp) 102.05
Sequencing read coverage depth 209.9x
# of contigs 1287
# of scaffolds 1287
# of scaffolds >= 2Kbp 1280
Scaffold N50 283
Scaffold L50 (Mbp) 0.13
# of gaps 0
% of scaffold length in gaps 0.0%
Three largest Scaffolds (Mbp) 0.46, 0.44, 0.39

Please note that this RNAseq library suffers from high rRNA contamination with ~60% of reads matching the SILVA database.
ESTs Data set # sequences total # mapped to genome % mapped to genome
Ests est.fasta 228739513 42211580 18.5%
Other Trinity_assembled_Illumina_transcriptome 64592 60741 94.0%

Gene Models FilteredModels2
length (bp) of: average median
gene 1631 1366
transcript 1403 1167
exon 338 165
intron 74 65
protein length (aa) 395 317
exons per gene 4.15 3
# of gene models 14257



The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.