Status
[February 2021] The Lentinula aciculospora JLM2183 v1.0 genome was sequenced with PacBio, assembled with HiCanu, and annotated with the JGI Annotation Pipeline. Mitochondrial genome was assembled separately and is available in the downloads section.
The genome is likely a polymorphic dikaryon, and this is reflected in an assembly and annotation with significant separation of alleles. Many of the scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertiore of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.
Genome Assembly | |
Genome Assembly size (Mbp) | 110.95 |
Sequencing read coverage depth | 19.29x |
# of contigs | 290 |
# of scaffolds | 290 |
# of scaffolds >= 2Kbp | 290 |
Scaffold N50 | 25 |
Scaffold L50 (Mbp) | 1.19 |
# of gaps | 0 |
% of scaffold length in gaps | 0.0% |
Three largest Scaffolds (Mbp) | 5.75, 4.35, 4.27 |
ESTs | Data set | # sequences total | # mapped to genome | % mapped to genome |
EstClusters | ESTclusters | 67963 | 60140 | 88.5% |
Ests | est.fasta | 425647019 | 421526312 | 99.0% |
Gene Models | FilteredModels2 | |
length (bp) of: | average | median |
gene | 1888 | 1635 |
transcript | 1535 | 1316 |
exon | 235 | 142 |
intron | 66 | 57 |
description: | ||
protein length (aa) | 447 | 366 |
exons per gene | 6.53 | 5 |
# of gene models | 11469 |
Collaborators
- David Hibbett, Clark University
Genome Reference(s)
Sierra-Patev Sean, Min Byoungnam, Naranjo-Ortiz Miguel, Looney Brian, Konkel Zachary, Slot Jason C., Sakamoto Yuichi, Steenwyk Jacob L., Rokas Antonis, Carro Juan, Camarero Susana, Ferreira Patricia, Molpeceres Gonzalo, Ruiz-Dueñas Francisco J., Serrano Ana, Henrissat Bernard, Drula Elodie, Hughes Karen W., Mata Juan L., Ishikawa Noemia Kazue, Vargas-Isla Ruby, Ushijima Shuji, Smith Chris A., Donoghue John, Ahrendt Steven, Andreopoulos William, He Guifen, LaButti Kurt, Lipzen Anna, Ng Vivian, Riley Robert, Sandor Laura, Barry Kerrie, MartÃnez Angel T., Xiao Yang, Gibbons John G., Terashima Kazuhisa, Grigoriev Igor V., Hibbett David
A global phylogenomic analysis of the shiitake genus <i>Lentinula</i>
Proc. Natl. Acad. Sci. U.S.A.. 2023;120(10): doi: 10.1073/pnas.2214076120
Links
- JGI PhyloGroup Portals: Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricales Lentinula
- JGI White Rot Fungi EcoGroup Portal