Status
(Jun 2016) The Laccaria bicolor S238N 93.12 genome was sequenced using PacBio, assembled using Falcon, and annotated using the JGI Annotation Pipeline. The transcriptome was sequenced using Illumina and assembled using Trinity.
Laccaria bicolor S238N 93.12 is likely a polymorphic dikaryon, and this is reflected in an assembly and annotation with significant separation of alleles. 412 of the 614 scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertiore of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.
Genome Assembly | |
Genome Assembly size (Mbp) | 96.43 |
Sequencing read coverage depth | |
# of contigs | 1034 |
# of scaffolds | 1034 |
# of scaffolds >= 2Kbp | 1028 |
Scaffold N50 | 109 |
Scaffold L50 (Mbp) | 0.24 |
# of gaps | 0 |
% of scaffold length in gaps | 0.0% |
Three largest Scaffolds (Mbp) | 1.47, 1.41, 1.05 |
ESTs | Data set | # sequences total | # mapped to genome | % mapped to genome |
Ests | est.fasta | 124840611 | 122511749 | 98.1% |
Other | JGI_RNA_contigs | 101217 | 87795 | 86.7% |
Gene Models | FilteredModels1 | |
length (bp) of: | average | median |
gene | 1456 | 1153 |
transcript | 1137 | 882 |
exon | 220 | 140 |
intron | 78 | 56 |
description: | ||
protein length (aa) | 341 | 251 |
exons per gene | 5.17 | 4 |
# of gene models | 25412 |
Collaborators
- DOE Joint Genome Institute
- Francis Martin at MycorWeb
- Annegret Kohler at MycorWeb