Info • Morchella brunnea NRRL 20869 v1.0


[April 2020] The Morchella brunnea Mel-22 v1.0 genome was sequenced with PacBio, assembled with Falcon, and annotated with the JGI Annotation Pipeline. Mitochondrial genome was assembled separately and is available in the downloads section.

We identified polymorphic content, reflected in an assembly and annotation with significant separation of alleles. Many of the scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertiore of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.

Summary statistics for the Morchella brunnea Mel-22 v1.0 release are below.
Genome Assembly
Genome Assembly size (Mbp) 56.69
Sequencing read coverage depth 140.36x
# of contigs 138
# of scaffolds 138
# of scaffolds >= 2Kbp 138
Scaffold N50 13
Scaffold L50 (Mbp) 1.66
# of gaps 0
% of scaffold length in gaps 0.0%
Three largest Scaffolds (Mbp) 4.25, 3.79, 2.41

ESTs Data set # sequences total # mapped to genome % mapped to genome
EstClusters ESTclusters 54758 52667 96.2%
Ests est.fasta 82113882 81095602 98.8%

Gene Models FilteredModels2
length (bp) of: average median
gene 1818 1570
transcript 1580 1337
exon 433 275
intron 92 67
protein length (aa) 448 359
exons per gene 3.65 3
# of gene models 10595



The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.