The genome of Mycena leptocephala was sequenced as part of the Mycenaceae sequencing project and the overarching JGI 1000 Fungal Genomes project “Deep Sequencing of Ecologically-relevant Dikarya“ (CSP 1974).This project will examine members of the Mycena genus to evaluate the genomic basis of their different nutritional modes.
The nitrous bonnet, Mycena
leptocephala
Mycena leptocephala is a member of the large
Fragilipedes section (Maas Geesteranus 1988, Aronsen and
Læssøe 2016). It can generally be recognised by having
a translucent-striated pileus with brownish colours and a
white/whitish margin, a similarly coloured stipe usually with white
fibrils, and a nitrous smell. It grows on a wide variety of
substrates, including grass and woody debris, and it has been
recorded from a multitude of places in both hemispheres. The
collection for this sequencing was found in Bolterdalen at Svalbard
on the 11th of August 2018. Besides its versatile ecology, this
species was chosen for genomic sequencing because it could be found
in the Arctic where the extreme environmental conditions may drive
unusual ecology patterns and genome sizes.
This genome was derived from dikaryotic (diploid) pure culture on
MEA agar with ampicilin and benomyl and should be free of
xenobiotic contaminations. Researchers who wish to use data from
unpublished Mycena genomes for publication are
respectfully required to contact the PI and JGI to avoid potential
conflicts on data use and coordinate other publications with the
Mycena master paper(s).
References
Aronsen, A.; Læssøe, T.: The genus Mycena
s.l. in The Fungi of Northern Europe, vol. 5. Copenhagen, 373 p
(2016).
Maas Geesteranus, R. A. (1988): Conspectus of the Mycenas
of the Northern Hemisphere. Proc. Kon. Ned. Akad. v. Wetensch.
(Ser. C).
Genome Reference(s)
Harder CB, Miyauchi S, Virágh M, Kuo A, Thoen E, Andreopoulos B, Lu D, Skrede I, Drula E, Henrissat B, Morin E, Kohler A, Barry K, LaButti K, Salamov A, Lipzen A, Merényi Z, Hegedüs B, Baldrian P, Stursova M, Weitz H, Taylor A, Koriabine M, Savage E, Grigoriev IV, Nagy LG, Martin F, Kauserud H
Extreme overall mushroom genome expansion in Mycena s.s. irrespective of plant hosts or substrate specializations.
Cell Genom. 2024 Jun 19;():100586. doi: 10.1016/j.xgen.2024.100586