Mycena capillaripes fruiting bodies on conifer litter (credit: Hervé Cochard, INRA)

The genome of Mycena capillaripes was sequenced as part of the overarching JGI 1000 Fungal Genomes project "Deep Sequencing of Ecologically-relevant Dikarya" (CSP 1974) - and more specifically, as a part of the Mycenaceae sequencing project. This project will examine members of the Mycena genus with respect to evaluate the genomic basis of their different nutritional modes.

The Pinkedge Bonnet, Mycena capillaripes

Mycena capillaripes is a member of the rubromarginatae section of the Mycena family, and closely related to Mycena rubromarginata (Smith 1936, Maas Geesteranus 1988). Indeed, the culture from which the genome is sequenced was originally misidentified as M. rubromarginata. This was collected in England, and the species has been reported from all over Europe and North America (Maas Geesteranus 1988), but does not appear to be common anywhere, though it might well be overlooked due to misidentification.

Pileus 5-25 mm across, conical, obtusely conical, campanulate or parabolical, at age sometimes somewhat depressed centrally, pruinose, glabrescent, shallowly sulcate, translucent-striate, hygrophanous, pale grey to greyish brown, somewhat darker (often reddish) at the centre, the margin often very pale, pallescent when drying. Lamellae 12-20 reaching the stipe, ascending, narrowly adnate, dorsally intervenose with age, whitish, cream to grey, the sides densely punctate with minute, dark red-brown dots, the edge dark reddish brown. Stipe 20-70 x 1-1.8 mm, hollow, straight to curved, terete, fragile, glabrous except for the pruinose apex, becoming shiny, grey, greyish brown, darker below, the apex whitish to grey; the base covered with white fibrils. Odour nitrous. See also: http://www.mycena.no/capillaripes.htm.

It is generally confined to coniferous needles, but can occasionally be found on litter of deciduous trees. Growing season is autumn. It is related to M. olivaceomarginata and M. albidolilacea, which will also be sequenced as part of the Mycenaceae project. As these two others grow in grass and moss, the sequencing of this species will yield important information about the evolution of adaptive niche differences among closely related species.

This genome was derived from diploid pure culture on MEA agar with ampicilin and benomyl and should be free of xenobiotic contaminations.

Researchers who wish to publish analyses using data from unpublished Mycena genomes are respectfully required to contact the PI and JGI to avoid potential conflicts on data use and coordinate other publications with the Mycena master paper(s).

References

Maas Geesteranus, R. A. (1988): Conspectus of the Mycenas of the Northern Hemisphere. Proc. Kon. Ned. Akad. v. Wetensch. (Ser. C).

Smith, Alexander H. "Studies in the Genus Mycena. III." Mycologia 28.5 (1936): 410-430.