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Mycena vitilis
The actual collection that has been cultured and subsequently sequenced. Photo credit: Christoffer Bugge Harder and Thomas Læssøe.

The genome of Mycena vitilis was sequenced as part of the Mycenaceae sequencing project and the overarching JGI 1000 Fungal Genomes project “Deep Sequencing of Ecologically-relevant Dikarya“ (CSP 1974). This project will examine members of the Mycena genus to evaluate the genomic basis of their different nutritional modes.

The snapping bonnet, Mycena vitilis

Mycena vitilis is a member of the large Fragilipedes section (Maas Geesteranus 1988) and is fairly common on litter and debris of hardwood, more rarely on coniferous ditto (Aronsen and Læssøe 2016, Robich 2016). It is widely found in Europe and has been reported from North America (Smith 1947) and North Asia, too.

Pileus 6-22 mm across, conical to campanulate, flattening with age, translucent-striate, sulcate, glabrous, somewhat lubricous when wet, becoming shiny when dry, grey-brown to pale grey, darker at the centre, paler to almost white at the margin. Lamellae 14-23 reaching the stipe, ascending, narrowly adnate to almost free, smooth but becoming veined to heavily ribbed with age, dorsally intervenose, white to whitish, sometimes more brownish, the edge concolorous to white. Stipe 30-80 x 1-2 mm, hollow, elastic-firm or even tenacious when wet, cartilaginous-brittle when dry, equal to somewhat widening below, terete, straight to curved, pruinose above, glabrous farther down, lubricous to almost viscid when wet, shiny when dry, pale grey-brown, usually with a whitish apex, sometimes with reddish brown spots below, the base sometimes rooting, densely covered with long, coarse, flexuous, whitish fibrils. Odour indistinctive.

M. vitilis has been shown to produce the antifungal agent strobilurin B as a competition mechanism against other fungi (Rahman 2006), and we expect that its genome will provide the foundation for identifying the pathway that leads to the production of strobilurin B. Besides, it has a lifestyle of an ”intermediate” generalist species that is able to grow on conifer wood debris but prefers hardwood, and will as thus fit well into our general analysis of the evolution of the lifestyle of Mycena.

This genome was derived from monokaryotic (haploid) pure culture on MEA agar with ampicilin and benomyl and should be free of xenobiotic contaminations. Researchers who wish to use data from unpublished Mycena genomes for publication are respectfully required to contact the PI and JGI to avoid potential conflicts on data use and coordinate other publications with the Mycena master paper(s).

References

Aronsen, A.; Læssøe, T.: The genus Mycena s.l. in The Fungi of Northern Europe, vol. 5. Copenhagen, 373 p (2016).

Maas Geesteranus, R. A. (1988): Conspectus of the Mycenas of the Northern Hemisphere. Proc. Kon. Ned. Akad. v. Wetensch. (Ser. C).

Rahman A-U. (2006). Bioactive Natural Products. Studies in Natural Product Chemistry. 32. p. 510. ISBN 978-0-444-52171-2.

Robich, G: Mycena d'Europa vol. 2. Centro Studi Micologici. A.M.B, Trento, p. 733-1528 (2016)

Smith AH. (1947). North American species of Mycena. Ann Arbor: University of Michigan Press.