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Nannochloropsis oceanica CCAP849/10
Nannochloropsis oceanica CCAP849/10 was imaged using a Keyence BZ-X700 with 100x oil immersion PlanApo NA 1.45 objective lens and overlayed with chlorophyll autofluorescence (Cy5) (bandwidths: excitation 620/60, emission 700/75). Image credit: Brandon Rohnke, Peers Lab at Colorado State University.

The Nannochloropsis oceanica CCAP849/10 genome sequence and gene models were not determined by the Joint Genome Institute (JGI), but were provided by collaborators at Los Alamos National Laboratory (LANL). In order to ensure this genome is comparable to those sequenced by the JGI, we applied filters to remove if present: 1) transposable elements, 2) pseudogenes, 3) alternative transcripts and overlapping models, 4) alleles on secondary scaffolds and 5) unsupported short models. This resulted in the removal of 1,087 models and the generation of the FilteredModels1 (GeneCatalog) gene track. All published models are available in the ExternalModels track. Please note that this copy of the genome is not maintained by LANL and is therefore not automatically updated. In order to allow comparative analyses with other algal genomes sequenced by the JGI, a copy of this genome is incorporated into PhycoCosm. The JGI Annotation Pipeline was used to add functional annotation to this genome.

Note: If you intend to publish any articles including Nannochloropsis oceanica CCAP849/10 genomic data, please contact Shawn Starkenburg ([email protected]) for permission prior to publication.

Nannochloropsis oceanica CCAP849/10

Nannochloropsis oceanica is an industrially relevant unicellular microalgal Eugstigmatophyte [1,2]. Molecular protocol development for N. oceanica has indicated successful application of CRISPR/Cas9 genome editing, targeted gene replacement, and transformation capabilities [3-6]. N. oceanica CCAP849/10 was obtained from the Culture Collection of Algae and Protozoa (CCAP) from the United Kingdom and is easily cultivated in f/2 medium.

References:

  1. Zienkiewicz, K., Zienkiewicz, A., Poliner, E. et al. Nannochloropsis, a rich source of diacylglycerol acyltransferases for engineering of triacylglycerol content in different hosts. Biotechnol Biofuels 10, 8 (2017). https://doi.org/10.1186/s13068-016-0686-8
  2. Guerra I, Pereira H, Costa M, Silva JT, Santos T, Varela J, Mateus M, Silva J. Operation Regimes: A Comparison Based on Nannochloropsis oceanica Biomass and Lipid Productivity. Energies. 2021; 14(6):1542. https://doi.org/10.3390/en14061542
  3. Osorio, H., Jara, C., Fuenzalida, K. et al. High-efficiency nuclear transformation of the microalgae Nannochloropsis oceanica using Tn5 Transposome for the generation of altered lipid accumulation phenotypes. Biotechnol Biofuels 12, 134 (2019). https://doi.org/10.1186/s13068-019-1475-y
  4. Vieler A, Wu G, Tsai CH, Bullard B, Cornish AJ, et al. (2012) Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga Nannochloropsis oceanica CCMP1779. PLOS Genetics 8(11): e1003064. https://doi.org/10.1371/journal.pgen.1003064
  5. Zhang, Z., Guo, L., Liu, H. et al. Zeocin treatment significantly elevated transformation efficiency of Nannochloropsis oceanica. J Appl Phycol 34, 1587–1594 (2022). https://doi.org/10.1007/s10811-022-02703-4
  6. Mihris Ibnu Saleem Naduthodi, Christian Südfeld, Emmanouil Klimis Avitzigiannis, Nicola Trevisan, Eduard van Lith, Javier Alcaide Sancho, Sarah D’Adamo, Maria Barbosa, and John van der Oost, Comprehensive Genome Engineering Toolbox for Microalgae Nannochloropsis oceanica Based on CRISPR-Cas Systems. ACS Synthetic Biology 2021 10 (12), 3369-3378 DOI: 10.1021/acssynbio.1c00329