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Home • Obba rivulosa 3A-2 v1.0
Obba rivulosa by Otto Miettinen
Obba rivulosa by Otto Miettinen

Obba rivulosa (also known as Ceriporiopsis rivulosa, Physisporinus rivulosus) is a basidiomycete that produces effused fruiting bodies with a poroid hymenophore. It belongs to the Polyporales, and is closely related with Gelatoporia subvermispora (= Ceriporiopsis subvermispora). The species is widespread in the temperate zone of the Northern Hemisphere, but it is rare throughout most of its range. It causes white rot on fallen logs of conifers and has been repeatedly collected on burned wood. The North American and European populations of this species show slight genetic variation (Miettinen & Rajchenberg 2012). This genome is based on a strain from Finland, North Europe, isolated from a burned softwood log in a recently burned forest area.

During wood degradation Obba rivulosa degrades lignin selectively, making it a particularly interesting fungus for studying enzymatic machinery of white-rot. The mechanism behind lignin degradation is not understood fully, and study of selective delignifiers will be highly useful in advancing that understanding. Selective delignification is potentially a useful character in biotechnological applications. In a large research project aiming to find suitable fungi for spruce biopulping Obba rivulosa appeared to be the most promising fungus (Hatakka et al. 2003, Hakala et al. 2004, Maijala et al. 2008). It is also promising fungus for bioremediation of old saw mill soils containing chlorinated compounds (Valentin et al. 2013). Manganese peroxidases and laccases of O. rivulosa have been characterized (Hakala et al. 2006, Hildén et al. 2007, 2013).

Obba rivulosa and Gelatoporia subvermispora are both selecive delignifiers. Results of this genome sequencing will be used to compare genomes and transcriptomes of these two species against each other as well as against other, non-selective white-rot fungi.

The Obba genome project is coordinated by Otto Miettinen (University of Helsinki). Most work will be conducted in the lab of Annele Hatakka and Kristiina Hildén (Univ. Helsinki). Research partners include also Dan Cullen (USDA FPL) and David Hibbett (Clark University). This project is part of the 1000 fungal genomes project.

Genome Reference(s)

References

Hatakka, A., Maijala, P., Hakala, T., Hauhio, L. & Ellmén, J. 2003. A new white-rot fungus and its use in the pretreatment of wood. Finnish patent FI 112248. November 14, 2003.

 

Hakala, T.K., Maijala, P., Konn, J. & Hatakka, A.  2004. Evaluation of novel wood-rotting polypore and corticioid fungi for the decay and biopulping of Norway spruce (Picea abies) wood. Enzyme and Microbial Technology 34:255-263.

 

Hakala, T.K., Hildén, K., Maijala, P., Olsson, C. & Hatakka, A. 2006. Differential regulation of manganese peroxidases and characterization of two variable MnP encoding genes in the white-rot fungus Physisporinus rivulosus. Applied Microbiology and Biotechnology 73:839-849.

 

Hildén, K.S., Hakala, T.K., Maijala, P., Lundell, T. & Hatakka, A. 2007. Novel thermotolerant laccases produced by the white-rot fungus Physisporinus rivulosus. Applied Microbiology and Biotechnology 77:301-309.

 

Maijala, P., Kleen, M., Westin, C., Poppius-Levlin, K., Herranen, K., Lehto, J.H., Reponen, P., Mäentausta, O., Mettälä, A. & Hatakka, A. 2008. Biomechanical pulping of softwood with enzymes and the white-rot fungus Physisporinus rivulosus. Enzyme and Microbial Technology 43:169–177.

 

Hildén, K.S., Mäkelä, M.R.,  Lundell, T., Kuuskeri, J.,  Chernykh, A., Golovleva, L., Archer, D. & Hatakka, A.  2013. Heterologous expression and structural characterization of two low pH laccases from a biopulping white-rot fungus Physisporinus rivulosus. Applied Microbiology and Biotechnology 97:1589–1599.

 

Miettinen, O., Rajchenberg, M. 2012: Obba and Sebipora, new polypore genera related to Cinereomyces and Gelatoporia (Polyporales, Basidiomycota). Mycological Progress 11: 131-147.

 

Valentín, L., Oesch-Kuisma, H., Steffen, K.T., Kähkönen, M.A., Hatakka, A. & Tuomela, M. 2013. Mycoremediation of wood and soil from an old sawmill area contaminated since decades. Journal of Hazardous Materials 260: 668– 675.