[June 2020] Chromatin capture data (Hi-C) was generated by Phase genomics for the sequencing of the Piromyces sp. UH3-1 genome. The draft assembly of Piromyces sp. UH3-1 generated using PacBio (Piromy1), was then used along with PacBio read data to purify haplotypes using FALCON-Phase (Kronenberg et al., 2019). Phase Genomics' Hi-C genome scaffolding platform was then used to create chromosome-scale scaffolds (generated by Kyle Langford at Phase Genomics), followed by error correction using Juicebox (Neva et al., 2016, Suhas et al., 2014). The final scaffolds included 12 chromosomes for Piromyces sp. UH3-1 (spanning 83.4 Mbp) with an N50 of 7.3 Mbp. While most of the assembly is contained within these 12 scaffolds, unplaced scaffolds were additionally included here for annotation using the JGI annotation pipeline.