Info • Pisolithus marmoratus ssp. 2 16A v1.0


[August 2018] (August 2018) The Pisolithus marmoratus genome was sequenced with PacBio, assembled with Falcon, and annotated with the JGI Annotation Pipeline. The transcriptome was sequenced with Illumina and assembled with Trinity. The mitochondrial genome was assembled separately and is available on the Download page.

The genome is likely a polymorphic dikaryon, and this is reflected in an assembly and annotation with significant separation of alleles. Many of the scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertiore of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.

Summary statistics for the Pisolithus marmoratus ssp. 2 16A v1.0 release are below.
Genome Assembly
Genome Assembly size (Mbp) 122.38
Sequencing read coverage depth 75.38x
# of contigs 1188
# of scaffolds 1188
# of scaffolds >= 2Kbp 1177
Scaffold N50 172
Scaffold L50 (Mbp) 0.20
# of gaps 0
% of scaffold length in gaps 0.0%
Three largest Scaffolds (Mbp) 1.25, 1.13, 0.87

ESTs Data set # sequences total # mapped to genome % mapped to genome
EstClusters ESTclusters 149475 130304 87.2%
Ests est.fasta 156906455 155515807 99.1%

Gene Models FilteredModels2
length (bp) of: average median
gene 1589 1301
transcript 1249 1002
exon 216 139
intron 73 56
protein length (aa) 370 285
exons per gene 5.79 5
# of gene models 21754


The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.