Status
[May 2019] The Polychytrium aggregatum JEL109 v1.0 genome was sequenced with PacBio, assembled with Falcon, and annotated with the JGI Annotation Pipeline. Mitochondrial genome was assembled separately and is available in the downloads section.
This genome is likely diploid, and this is reflected in an assembly and annotation with significant separation of alleles. Many of the scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertiore of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.
Genome Assembly | |
Genome Assembly size (Mbp) | 64.92 |
Sequencing read coverage depth | 279.61x |
# of contigs | 461 |
# of scaffolds | 461 |
# of scaffolds >= 2Kbp | 457 |
Scaffold N50 | 41 |
Scaffold L50 (Mbp) | 0.39 |
# of gaps | 0 |
% of scaffold length in gaps | 0.0% |
Three largest Scaffolds (Mbp) | 1.62, 1.46, 1.42 |
ESTs | Data set | # sequences total | # mapped to genome | % mapped to genome |
EstClusters | ESTclusters | 29833 | 26279 | 88.1% |
Ests | est.fasta | 64266015 | 62436535 | 97.2% |
Gene Models | FilteredModels1 | |
length (bp) of: | average | median |
gene | 2435 | 2035 |
transcript | 1698 | 1423 |
exon | 312 | 173 |
intron | 167 | 148 |
description: | ||
protein length (aa) | 486 | 386 |
exons per gene | 5.45 | 4 |
# of gene models | 10690 |
Collaborators
- Tim James, University of Michigan, USA
- Joseph Spatafora, Oregon State University, USA
Genome Reference(s)
Amses KR, Simmons DR, Longcore JE, Mondo SJ, Seto K, Jerônimo GH, Bonds AE, Quandt CA, Davis WJ, Chang Y, Federici BA, Kuo A, LaButti K, Pangilinan J, Andreopoulos W, Tritt A, Riley R, Hundley H, Johnson J, Lipzen A, Barry K, Lang BF, Cuomo CA, Buchler NE, Grigoriev IV, Spatafora JW, Stajich JE, James TY
Diploid-dominant life cycles characterize the early evolution of Fungi.
Proc Natl Acad Sci U S A. 2022 Sep 6;119(36):e2116841119. doi: 10.1073/pnas.2116841119
Links
- 1000 Fungal Genomes Project
- JGI PhyloGroup Portals: Fungi Chytridiomycota Chytridiomycetes