Status
[December 2015] The Rhizoclosmatium globosum JEL800
genome was sequenced using the PacBio platform and assembled
together with Falcon version 20150310
(https://github.com/PacificBiosciences/FALCON) and improved with
finisherSC version 2.0 (Lam et al., 2014), then polished with
Quiver version
smrtanalysis_2.3.0.140936 (Chin et al., 2013).
Rhizoclosmatium hyalinus JEL800 was determined to be a diploid/heterokaryon assembly. 326 of the 437 scaffolds (40.4% of total assembly) displayed significant similarity to larger scaffolds and thus were predicted to belong to the alternate haplotype. These have been designated 'secondary scaffolds'. To be a secondary scaffold, >50% of the scaffold had to be at least 95% identical to a larger scaffold. In the case of Rhizoclosmatium, on average 91.3% of the secondary scaffold was at least 95% identical to a larger scaffold. As a result, we created two new tracks, the 'primary alleles' and 'secondary alleles' tracks, which highlight the models which are found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertiore of the genome, and thus models unique to the secondary scaffolds were included in the GC, while others were removed.
Genome Assembly | |
Genome Assembly size (Mbp) | 57.02 |
Sequencing read coverage depth | |
# of contigs | 437 |
# of scaffolds | 437 |
# of scaffolds >= 2Kbp | 435 |
Scaffold N50 | 51 |
Scaffold L50 (Mbp) | 0.29 |
# of gaps | 0 |
% of scaffold length in gaps | 0.0% |
Three largest Scaffolds (Mbp) | 1.02, 0.95, 0.94 |
ESTs | Data set | # sequences total | # mapped to genome | % mapped to genome |
Ests | RNNotator_assembled_Illumina_ESTs | 40307 | 38300 | 95.0% |
Gene Models | FilteredModels1 | |
length (bp) of: | average | median |
gene | 1530 | 1235 |
transcript | 1319 | 1070 |
exon | 330 | 211 |
intron | 73 | 55 |
description: | ||
protein length (aa) | 395 | 312 |
exons per gene | 4.00 | 3 |
# of gene models | 16990 |
Collaborators
Jason Stajich, UC Riverside, California
Joey Spatafora, Oregon State, Oregon
Genome Reference(s)
Mondo SJ, Dannebaum RO, Kuo RC, Louie KB, Bewick AJ, LaButti K, Haridas S, Kuo A, Salamov A, Ahrendt SR, Lau R, Bowen BP, Lipzen A, Sullivan W, Andreopoulos BB, Clum A, Lindquist E, Daum C, Northen TR, Kunde-Ramamoorthy G, Schmitz RJ, Gryganskyi A, Culley D, Magnuson J, James TY, O'Malley MA, Stajich JE, Spatafora JW, Visel A, Grigoriev IV
Widespread adenine N6-methylation of active genes in fungi.
Nat Genet. 2017 Jun;49(6):964-968. doi: 10.1038/ng.3859