Info • Russula sp. SA2 v1.0

Status

[October 2022] The Russula sp. SA2 v1.0 genome was sequenced with PacBio, assembled with Flye, and annotated with the JGI Annotation Pipeline. The mitochondrial genome was assembled separately and is available in the downloads section.

This genome is likely a polymorphic dikaryon, and this is reflected in an assembly and annotation with significant separation of alleles. Some of the scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertoire of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.

Genome Assembly
Genome Assembly size (Mbp) 72.28
Sequencing read coverage depth 39.26x
# of contigs 280
# of scaffolds 280
# of scaffolds >= 2Kbp 280
Scaffold N50 40
Scaffold L50 (Mbp) 0.61
# of gaps 0
% of scaffold length in gaps 0.0%
Three largest Scaffolds (Mbp) 1.76, 1.74, 1.55


ESTs Data set # sequences total # mapped to genome % mapped to genome
EstClusters ESTclusters 86368 75588 87.5%
Ests est.fasta 116258444 115407558 99.3%


Gene Models FilteredModels1
length (bp) of: average median
gene 2037 1696
transcript 1666 1374
exon 262 161
intron 71 58
description:
protein length (aa) 483 376
exons per gene 6.36 5
# of gene models 11878


Funding

The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.