Info • Russula emetica 2018DUKE113 v1.0

Status

[November 2022] The Russula emetica 2018DUKE113 v1.0 genome was sequenced with PacBio, assembled with Flye, and annotated with the JGI Annotation Pipeline. Mitochondrial genome was assembled separately and is available in the downloads section.

This genome is likely a polymorphic dikaryon, and this is reflected in an assembly and annotation with significant separation of alleles. Some of the scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertoire of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.

Genome Assembly
Genome Assembly size (Mbp) 73.76
Sequencing read coverage depth 31.4x
# of contigs 378
# of scaffolds 378
# of scaffolds >= 2Kbp 378
Scaffold N50 64
Scaffold L50 (Mbp) 0.41
# of gaps 0
% of scaffold length in gaps 0.0%
Three largest Scaffolds (Mbp) 1.33, 0.98, 0.89


Note: RNA has some contamination, resulting in a lower-than-exoected assembly mapping rate.
ESTs Data set # sequences total # mapped to genome % mapped to genome
EstClusters ESTclusters 122932 95726 77.9%
Ests est.fasta 194058249 192849339 99.4%


Gene Models FilteredModels1
length (bp) of: average median
gene 1993 1669
transcript 1632 1351
exon 264 160
intron 72 58
description:
protein length (aa) 484 382
exons per gene 6.17 5
# of gene models 13473


Funding

The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.