Info • Stropharia rugosoannulata MYCOTA018 v1.0

Status

[July 2022] The Stropharia rugosoannulata MYCOTA018 v1.0 genome was sequenced with PacBio, assembled with Flye, and annotated with the JGI Annotation Pipeline. The transcriptome was sequenced with Illumina and assembled with Trinity.  Mitochondrial genome was assembled separately and is available in the downloads section.

This genome is likely a polymorphic dikaryon, and this is reflected in an assembly and annotation with significant separation of alleles. Some of the scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertoire of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.

Genome Assembly
Genome Assembly size (Mbp) 96.79
Sequencing read coverage depth 50.5x
# of contigs 388
# of scaffolds 388
# of scaffolds >= 2Kbp 388
Scaffold N50 56
Scaffold L50 (Mbp) 0.56
# of gaps 0
% of scaffold length in gaps 0.0%
Three largest Scaffolds (Mbp) 2.03, 1.80, 1.44


ESTs Data set # sequences total # mapped to genome % mapped to genome
EstClusters ESTclusters 71867 69479 96.7%
Ests est.fasta 175130177 173242631 98.9%


Gene Models FilteredModels1
length (bp) of: average median
gene 1897 1614
transcript 1530 1287
exon 249 150
intron 73 58
description:
protein length (aa) 449 360
exons per gene 6.15 5
# of gene models 17978


Funding

The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.