Home • Trichoderma reesei CBS999.97 MAT1-2
Photo of Trichoderma reesei CBS999.97 MAT1-2
Vegetative cultures of CBS999.97(MAT1-1) (A) and CBS999.97(MAT1-2) (B) on 9-cm Potato Dextrose Agar (PDA) plate under a 12-hour light and 12-hour dark photoperiod. Sexual crossing of CBS999.97(MAT1-1) and CBS999.97(MAT1-2) generates fruiting bodies with droplets of liquid forming on their surface (C). Visualization of developing and mature asci in the CBS999.97(MAT1-1)/CBS999.97(MAT1-2) fruiting bodies. Rosettes of asci were dissected from developing fruiting bodies, stained with Hoechst 33342 (2'-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole trihydrochloride trihydrate) and then visualized by fluorescence microscopy. Representative differential interference contrast (DIC) and Hoechst fluorescent images are shown (D-G). Developing asci at meiotic prophase (MP), the first meiotic nuclear division (MI) (D), the second meiotic nuclear division (MII) (E), the first postmeiotic mitosis (1st PM) (F) and the second postmeiotic mitosis (2nd PM) (G) as well as a mature ascus with 16 ascospores (sexual spores) (G) are indicated. Credits: Wan-Chen Li and Ting-Fang Wang. Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.

Trichoderma reesei (Hypocrea jecorina) CBS999.97 originates from French Guiana and is the first sexually competent wild isolate of Trichoderma spp. It is the teleomorph of T. reesei QM6a, the ancestor of all currently used cellulase-producing mutant. CBS999.97 (MAT1-1) and CBS999.97 (MAT1–2) were derived from two ascospores in a CBS999.97 fruiting body. With the support from Institute of Molecular Biology, Academia Sinica, Taiwan, Republic of China, the strain CBS999.97 was established as a new emerging model organism for studying fungal sexual development, segmental aneuploidy, repeat-induced point mutation, meiotic recombination, postmeiotic mitosis and mitochondrial inheritance.

The genome sequence and gene models of Trichoderma reesei CBS999.97 MAT1-2 were not determined by the Joint Genome Institute (JGI), but were downloaded from NCBI on Jan 29, 2020 and annotation provided by the authors. JGI tools were used to automatically annotate predicted proteins. Please note that this copy of the genome is not maintained by NCBI and is therefore not automatically updated.

Genome Reference(s)

Please cite the following publication(s) if you use the data from this genome in your research:

Li WC, Lee CY, Lan WH, Woo TT, Liu HC, Yeh HY, Chang HY, Chuang YC, Chen CY, Chuang CN, Chen CL, Hsueh YP, Li HW, Chi P, Wang TF
Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences.
Proc Natl Acad Sci U S A. 2021 Feb 23;118(8):. doi: 10.1073/pnas.2007192118

Li WC, Liu HC, Lin YJ, Tung SY, Wang TF
Third-generation sequencing-based mapping and visualization of single nucleotide polymorphism, meiotic recombination, illegitimate mutation and repeat-induced point mutation.
NAR Genom Bioinform. 2020 Sep;2(3):lqaa056. doi: 10.1093/nargab/lqaa056