Status
Assembly
v2.0 (May 2010). The assembly release version 2.0 is an improved assembly that has undergone gap closure and is now in 93 main genome contigs with no gaps. Roughly half of the genome is contained in 8 scaffolds all at least 1.8 MB in length.
v1.0 (Dec 2007): The assembly release version 1.0 of whole genome shotgun reads was constructed with the JGI assembler, JAZZ, using paired end sequencing reads at an Avgrage coverage of ~8.05X. After trimming for vector and quality, 445,647 reads assembled into 135 main genome scaffolds totaling 38.8 MB. Roughly half of the genome is contained in 9 scaffolds all at least 1.7 MB in length.
Nuclear Genome Assembly: | v1.0 | v2.0 |
Scaffold count: | 135 | 93 |
All Contig count: | 587 | 93 |
Scaffold sequence bases total: | 38.8 Mb | 39.0 Mb |
Scaffolded (Large) Contig sequence bases total: | 38.6 Mb | 39.0 Mb |
Estimated % sequence bases in gaps: | 0.4% | 0.0% |
Scaffold N50/L50: | 9/1.7 Mb | 8/1.8 Mb |
Contig N50/L50: | 66/173.9 Kb | 8/1.8 Mb |
Number of scaffolds > 50.0 Kb: | 48 | 43 |
% in scaffolds > 50.0 Kb: | 97.7% | 97.8% |
Annotation
v2.0 (May 2010): Annotation of the v2.0 assembly was produced by the JGI Annotation Pipeline, using a variety of homology-based and ab initio gene predictors. The v1.0 gene catalog along with its manual curations was mapped to the v2.0 assembly and included in filtering. After filtering for EST support, completeness and homology support, a total of 12,427 genes were structurally and functionally annotated.
v1.0 (Dec 2007) of the v1.0 assembly was produced by the JGI Annotation Pipeline, using a variety of homology-based and ab initio gene predictors. After filtering for EST support, completeness and homology support, a total of 11,655 genes were structurally and functionally annotated.
Nuclear Genome Annotation: | v1.0 | v2.0 |
# gene models: | 11,655 | 12,427 |
Gene density: | 300.39 | 318.64 |
Avg.gene length: | 1714 nt | 1711 nt |
Avg. protein length: | 480 aa | 471 aa |
Avg. exon frequency: | 2.98 exons/gene | 2.93 exons/gene |
Avg. exon length: | 507 nt | 515 nt |
Avg. intron length: | 104 nt | 106 nt |
% complete gene models (with start and stop codons): | 84% | 86% |
% genes with homology support: | 90% | 90% |
% genes with Pfam domains: | 54% | 61% |
Collaborators
- Chuck Kenerley, TAMU - Texas A and M University
- Heather Wilkinson, TAMU - Texas A and M University
- Dan Ebbole, TAMU - Texas A and M University
- Christian Kubicek, TU-Wien
- Alfredo Herrera-Estrella, Cinvestav
- Helena Nevalainen, Macquarie University
- Scott Baker, Pacific Northwest National Laboratory
- Ben Horwitz, Technio - Israel Institute of Technology
Genome Reference(s)
Kubicek CP, Herrera-Estrella A, Seidl-Seiboth V, Martinez DA, Druzhinina IS, Thon M, Zeilinger S, Casas-Flores S, Horwitz BA, Mukherjee PK, Mukherjee M, Kredics L, Alcaraz LD, Aerts A, Antal Z, Atanasova L, Cervantes-Badillo MG, Challacombe J, Chertkov O, McCluskey K, Coulpier F, Deshpande N, von Döhren H, Ebbole DJ, Esquivel-Naranjo EU, Fekete E, Flipphi M, Glaser F, Gómez-RodrÃguez EY, Gruber S, Han C, Henrissat B, Hermosa R, Hernández-Oñate M, Karaffa L, Kosti I, Le Crom S, Lindquist E, Lucas S, Lübeck M, Lübeck PS, Margeot A, Metz B, Misra M, Nevalainen H, Omann M, Packer N, Perrone G, Uresti-Rivera EE, Salamov A, Schmoll M, Seiboth B, Shapiro H, Sukno S, Tamayo-Ramos JA, Tisch D, Wiest A, Wilkinson HH, Zhang M, Coutinho PM, Kenerley CM, Monte E, Baker SE, Grigoriev IV
Comparative genome sequence analysis underscores mycoparasitism as the ancestral life style of Trichoderma.
Genome Biol. 2011;12(4):R40. doi: 10.1186/gb-2011-12-4-r40