Info • Phanerochaete chrysosporium v1.0
Please note that this organism is for archival use only. Please see the current Phanerochaete chrysosporium RP-78 v2.2 site for the latest data and information.
 
 
Status

This draft was generated using a whole genome shotgun method. The genomic DNA was purified from a homokaryotic strain RP-78 and five principal small insert (3-4 Kbp) pUC18 plasmid libraries were generated. Total 619,903 end sequences approximately 10.5X sequence coverage were produced. To further corroborate the long-range structure of the assembled genome, we end-sequenced 2000 clones from a cosmid library (1.5X clone coverage), and confirmed that the vast majority of end-sequences were found in opposing orientations within three standard deviations of each other.

 
Release
v1.0: A total of 619,903 paired sequence fragments (10.5X coverage) were assembled with the JGI assembler, JAZZ. The assembly contains 29.6 million base pairs of non-repetitive sequence in 349 scaffolds greater than 3 kbp. 90% of the assembly was in 165 scaffolds. 50% was found in 45 scaffolds longer than 203 Kbp. Gene modeling and analysis were performed at the JGI and ORNL.
Collaborators
  • Catholic University, Departmento de Genética Molecular y Microbiologíca, Facultad de Ciencias Biológicas, Pontifica Universidad Católica de Chile, Santiago, Chile and Millenium Institute for Fundamental and Applied (Santiago, Chile)

  • Department of Physics, University of California, Berkeley (Berkeley, CA, USA)

  • Novozyme Biotech, Inc. (Davis, CA, USA)

  • Oak Ridge National Laboratory (Oak Ridge, TN, USA)

  • Center National De La Recherche Scientifique (France)

  • USDA, Forest Service, Forest Products Laboratory (Madison, WI, USA)

  • Department of Biological Sciences, Columbia University (New York, NA USA)

Funding

This work was performed under the auspices of the US Department of Energy's Office of Science, Biological and Environmental Research Program and the by the University of California, Lawrence Livermore National Laboratory under Contract No. W-7405-Eng-48, Lawrence Berkeley National Laboratory under contract No. DE-AC03-76SF00098 and Los Alamos National Laboratory under contract No. W-7405-ENG-36.