Info • Amanita muscaria var. formosa 2016PMI152 v1.0

Status

[December 2020] The genome was sequenced with PacBio, assembled with HiCanu, and annotated with the JGI Annotation Pipeline. The transcriptome was sequenced with Illumina and assembled with Trinity. The mitochondrial genome was assembled separately and is available under the Download tab.

The genome is likely a polymorphic dikaryon, and this is reflected in an assembly and annotation with significant separation of alleles. Many of the scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertiore of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.

Genome Assembly
Genome Assembly size (Mbp) 110.26
Sequencing read coverage depth 38.46x
# of contigs 316
# of scaffolds 316
# of scaffolds >= 2Kbp 316
Scaffold N50 15
Scaffold L50 (Mbp) 2.17
# of gaps 0
% of scaffold length in gaps 0.0%
Three largest Scaffolds (Mbp) 5.89, 5.81, 4.58


ESTs Data set # sequences total # mapped to genome % mapped to genome
EstClusters ESTclusters 108351 103073 95.1%
Ests est.fasta 155516744 154965816 99.6%


Gene Models FilteredModels1
length (bp) of: average median
gene 1682 1426
transcript 1384 1165
exon 247 153
intron 67 55
description:
protein length (aa) 410 328
exons per gene 5.61 4
# of gene models 15793


Funding

The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.