Aspergillus ambiguus CBS 117.58 was sequenced as a part of the Aspergillus whole-genus sequencing project - a project dedicated to performing whole-genome sequencing of all members of the Aspergillus genus. The Aspergilli are a ubiquitous and species-rich genus, currently containing more than 300 filamentous fungi. The genus covers a wide range of phenotypes and has a substantial economic foot print, as it includes fermenters of foodstuffs, key cell factories for production of enzymes and organic acids, plant pathogens, model organisms for cell biology, human opportunistic pathogens, producers of animal and human mycotoxins, and degraders of a wide range of organic biomass relevant for bioenergy conversion.