Info • Boletus edulis Přilba v1.0


[December 2018] The genome was sequenced with PacBio, assembled with Falcon, and annotated with the JGI Annotation Pipeline. The transcriptome was sequenced with Illumina and assembled with Trinity. The mitochondrial genome was assembled separately and is available on the Download page.

Boletus edulis Přilba is likely a polymorphic dikaryon, and this is reflected in an assembly and annotation with significant separation of alleles. Many of the smaller scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertiore of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.

The B. edulis Přilba RNA sample was heavily contaminated, and so was supplemented with the B. edulis BED1 transcriptome ( The Přilba transcriptome accounts for most of the low RNA mapping rates.

Summary statistics for the Boletus edulis Přilba v1.0 release are below.
Genome Assembly
Genome Assembly size (Mbp) 69.19
Sequencing read coverage depth 173.85x
# of contigs 478
# of scaffolds 478
# of scaffolds >= 2Kbp 475
Scaffold N50 40
Scaffold L50 (Mbp) 0.38
# of gaps 0
% of scaffold length in gaps 0.0%
Three largest Scaffolds (Mbp) 2.96, 2.70, 2.23

ESTs Data set # sequences total # mapped to genome % mapped to genome
EstClusters ESTclusters 134951 54885 40.7%
Ests est.fasta 268355241 146976842 54.8%

Gene Models FilteredModels1
length (bp) of: average median
gene 1570 1287
transcript 1271 1016
exon 230 146
intron 68 56
protein length (aa) 380 291
exons per gene 5.53 4
# of gene models 18123

Genome Reference(s)


The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.