JF 03-3F
JF 03-3F is an unknown black yeast fungal culture whose highest hit on the NCBI database is Eurotiomycetes sp. Voucher ARIZ: SR0058 (KP991853.1); 97% matching. Black yeast fungi are recognized as polyextremotolerant fungi because of their high resistance to many stressors (Gostinčar et al., 2012). In addition, the name “black yeast fungi” refers to both their morphology and their highly melanized cell wall (Gostinčar et al., 2012). This black yeast was isolated as part of a broader survey of fungal-algal associations in dryland Biological Soil Crusts (BSCs). It was recovered from a BSC community located in Jackman Flats Provincial Park (B.C., Canada). JF 03-3F possesses a simplified morphology that does not include the presence of pseudohyphae or true hyphae. It does have difficulty spreading in water due to a highly hydrophobic cell surface. No teleomorphic state has yet been observed for JF 03-3F but that is typical of black yeast fungi (Untereriner et al., 1995). Extensive phenotyping shows that this isolate can use all standard nitrogen sources to support growth, but it is deficient in the use of several carbon sources. Moreover, JF 03-3 is resistant to several metals, but not nickel or cadmium. Its optimal growth temperature is 22°C, although it was able to grow at 27°C as well. Growth at 37°C, 42°C, and 4°C was severely stunted compared to 22°C, suggesting that growth above 27°C is not optimal.
References
• Gostinčar, C.,
Muggia, L., & Grube, M. (2012). Polyextremotolerant black
fungi: oligotrophism, adaptive potential, and a link to lichen
symbioses. Frontiers in microbiology, 3, 390.
• Untereiner, W. A.,
Straus, N. A., & Malloch, D. (1995). A
molecular-morphotaxonomic approach to the systematics of the
Herpotrichiellaceae and allied black yeasts. Mycological
Research, 99(8), 897-913.
Genome Reference(s)
Carr Erin C, Barton Quin, Grambo Sarah, Sullivan Mitchell, Renfro Cecile M, Kuo Alan, Pangilinan Jasmyn, Lipzen Anna, Keymanesh Keykhosrow, Savage Emily, Barry Kerrie, Grigoriev Igor V, Riekhof Wayne R, Harris Steven D
Characterization of a novel polyextremotolerant fungus, <i>Exophiala viscosa</i>, with insights into its melanin regulation and ecological niche
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