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Home • Morchella punctipes GB769 v1.0
Photo of Morchella punctipes GB769 v1.0
Figure 1. Image of Morchella punctipes collected in a mixed forest of Michigan USA. Image courtesy of Gregory Bonito.

In the "1KFG: Deep Sequencing of Ecologically-relevant Dikarya" project (CSP1974), we aim to sequence additional sampling of genomic diversity within keystone lineages of plant-interacting fungi and saprophytic fungi that are of special ecological importance for understanding terrestrial ecosystems. In addition, comparative genome analysis with saprotrophic, mycorrhizal and pathogenic fungi will provide new insights into the specific and conserved adaptations associated with each fungal lifestyle.

Within the framework of CSP1974, we are sequencing phylogenetically and morphologically diverse species of Morchellaceae. These fungi include economically important edible morels (Morchella), putatively toxic false-morels (Verpa), and the edible hypogeous truffle genera Leucangium and Kalapuya (1–3). The ecology of these taxa is still poorly understood. Some Morchella species are suspected to be mycorrhizal symbionts (4), others grow as endophytes within plant roots (5,6), some farm bacteria (7), but the majority of species (including the cultivated species of morels) are considered to be general saprotrophs (8,9). The Morchellaceae lineage is hypothesized to have originated and radiated in the Northern Hemisphere, later dispersing into Southern hemisphere regions (10). Genomic data generated by this project will be used to better identify genomic features underlying the distinct ecology, diversity, and morphology of Morchellaceae fungi.

Here we present the genome of Morchella punctipes (Peck, Bull), one of three species of fungi commonly known as ‘half-free’ morels, since the stipe attaches to the cap approximately half-way up the cap (Figure 1). This taxon corresponds to phylogenetic species Mel-4 as ascribed by O’Donnell et al. (2011) (10). Morchella punctipes is characterized by a stipe which is punctated with mealy granules that tend to darken with maturity on the surface. This species fruits from late March in southern regions to late May in northern regions. Morchella punctipes is widely distributed in hardwood forests east of the Rocky Mountains. This genome isolate (#GB769) was collected in Michigan, USA in 2017.

Researchers who wish to publish analyses using data from unpublished CSP genomes are respectfully required to contact the PI and JGI to avoid potential conflicts on data use and coordinate other publications with the CSP master paper(s).

 

References

  1. O’Donnell, K., Cigelnik, E., Weber, N. S. & Trappe, J. M. Phylogenetic Relationships among Ascomycetous Truffles and the True and False Morels Inferred from 18S and 28S Ribosomal DNA Sequence Analysis. Mycologia 89, 48–65 (1997).
  2. Gecan, J. S. & Cichowicz, S. M. Toxic Mushroom Contamination of Wild Mushrooms in Commercial Distribution. J. Food Prot. 56, 730–734 (1993).
  3. Trappe, M. J., Trappe, J. & Bonito, G. Kalapuya brunnea gen. & sp. nov. and its relationship to the other sequestrate genera in Morchellaceae. Mycologia 102, 1058–1065 (2010).
  4. Buscot, F. Mycorrhizal succession and morel biology. Mycorrhizas in ecosystems 220–224 (1992).
  5. Masaphy, S., Zabari, L., Goldberg, D. & Jander-Shagug, G. The complexity of Morchella systematics: a case of the yellow morel from Israel. Fungi 3, 14–18 (2010).
  6. Baynes, M., Newcombe, G., Dixon, L., Castlebury, L. & O’Donnell, K. A novel plant–fungal mutualism associated with fire. Fungal Biol. 116, 133–144 (2012).
  7. Pion, M., Spangenberg, J. E., Simon, A., Bindschedler, S., Flury, C., Chatelain, A., Bshary, R., Job, D. & Junier, P. Bacterial farming by the fungus Morchella crassipes. Proc. Biol. Sci. 280, 20132242 (2013).
  8. Benucci, G. M. N., Longley, R., Zhang, P., Zhao, Q., Bonito, G. & Yu, F. Microbial communities associated with the black morel Morchella sextelata cultivated in greenhouses. PeerJ 7, e7744 (2019).
  9. Hobbie, E. A., Rice, S. F., Weber, N. S. & Smith, J. E. Isotopic evidence indicates saprotrophy in post-fire Morchella in Oregon and Alaska. Mycologia 108, 638–645 (2016).
  10. O’Donnell, K., Rooney, A. P., Mills, G. L., Kuo, M., Weber, N. S. & Rehner, S. A. Phylogeny and historical biogeography of true morels (Morchella) reveals an early Cretaceous origin and high continental endemism and provincialism in the Holarctic. Fungal Genet. Biol. 48, 252–265 (2011).
  11. Kuo, M., Dewsbury, D. R., O'Donnell, K., Carter, M. C., Rehner, S. A., Moore, J. D., Moncalvo, J., Canfield, S. A., Stephenson, S. L., Methven, A. S., & Volk, T. J. Taxonomic revision of true morels (Morchella) in Canada and the United States, Mycologia, 104:5, 1159-1177 (2012).