The genome sequence and gene models of Nosema bombycis
CQ1 were not determined by the Joint Genome Institute (JGI), but
were downloaded from Ensembl
Fungi on April 11, 2020. Please note that this copy of the
genome is not maintained by Ensembl and is therefore not
automatically updated. The JGI Annotation Pipeline was used to add
additional functional annotation to the author's chromosomes and
proteins.
The microsporidian Nosema bombycis is the pathogen that
causes silkworm pébrine disease, which leads to enormous
economic losses to sericulture. N. bombyis is also the
first microsporidian species identified. Compared to other known
Nosema species, N. bombycis can unusually infect
a broad range of hosts. Besides horizontal transmission, N.
bombycis can also be vertically transmitted via invading into
insects ovary and embryo. The genome of N. bombycis was
estimated to be approximately 15.3 MB, organized in 18 chromosomes.
N. bombycis CQ1 (NbCQ1) is a strain isolated and
identified from infected silkworm in Chongqing, China. The genome
sequencing of the NbCQ1 was completed by the State Key Laboratory
of Silkworm Genome Biology, Southwest University. Genomic DNA and
mRNA of the NbCQ1 were extracted for constructing a plasmid library
with 1.5-2Kb inserts, a miniBAC library with 30-50Kb inserts, a
Solexa DNA library and cDNA libraries. In result, 6.7X Sanger reads
from the plasmid library, 0.4X miniBAC ends and ~28X Solexa reads
were sequenced for genome assembling and annotating. Finally, 1607
scaffolds with a total size of 15.8 MB were assembled from 3558
contigs, from which 4468 coding genes were predicted (Pan, et al,
2013). The genome of NbCQ1 was found to be high repetitive and
redundant for containing large quantities of gene duplications and
transposable elements, which led to the genome expansion of N.
bombycis.
Genome Reference(s)
Pan G, Xu J, Li T, Xia Q, Liu SL, Zhang G, Li S, Li C, Liu H, Yang L, Liu T, Zhang X, Wu Z, Fan W, Dang X, Xiang H, Tao M, Li Y, Hu J, Li Z, Lin L, Luo J, Geng L, Wang L, Long M, Wan Y, He N, Zhang Z, Lu C, Keeling PJ, Wang J, Xiang Z, Zhou Z
Comparative genomics of parasitic silkworm microsporidia reveal an association between genome expansion and host adaptation.
BMC Genomics. 2013 Mar 16;14():186. doi: 10.1186/1471-2164-14-186
References:
- Guoqing Pan et al. Comparative genomics of parasitic silkworm microsporidia reveal an association between genome expansion and host adaptation. BMC Genomics. 2013, 14: 186.
