Status
The Phakopsora pachyrhizi MG2006 v1.0 genome was sequenced with PacBio, assembled with MECAT, polished with arrow, and annotated with the JGI Annotation Pipeline. Mitochondrial genome was assembled separately and is available in the downloads section.
Phakopsora pachyrhizi is likely a polymorphic dikaryon, and this is reflected in an assembly and annotation with significant separation of alleles. Many of the smaller scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertiore of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.
Genome Assembly | |
Genome Assembly size (Mbp) | 1057.43 |
Sequencing read coverage depth | 142.83x |
# of contigs | 7465 |
# of scaffolds | 7464 |
# of scaffolds >= 2Kbp | 7464 |
Scaffold N50 | 1110 |
Scaffold L50 (Mbp) | 0.22 |
# of gaps | 1 |
% of scaffold length in gaps | 0.0% |
Three largest Scaffolds (Mbp) | 3.05, 2.57, 2.17 |
ESTs | Data set | # sequences total | # mapped to genome | % mapped to genome |
EstClusters | ESTclusters | 344612 | 130795 | 38.0% |
Ests | est.fasta | 1769119900 | 1451768142 | 82.1% |
Gene Models | FilteredModels2 | |
length (bp) of: | average | median |
gene | 1532 | 1223 |
transcript | 1066 | 783 |
exon | 234 | 135 |
intron | 133 | 94 |
description: | ||
protein length (aa) | 319 | 221 |
exons per gene | 4.56 | 4 |
# of gene models | 19618 |
Collaborators
Sébastien Duplessis, INRA, France.
Genome Reference(s)
Gupta YK, Marcelino-Guimarães FC, Lorrain C, Farmer A, Haridas S, Ferreira EGC, Lopes-Caitar VS, Oliveira LS, Morin E, Widdison S, Cameron C, Inoue Y, Thor K, Robinson K, Drula E, Henrissat B, LaButti K, Bini AMR, Paget E, Singan V, Daum C, Dorme C, van Hoek M, Janssen A, Chandat L, Tarriotte Y, Richardson J, Melo BDVA, Wittenberg AHJ, Schneiders H, Peyrard S, Zanardo LG, Holtman VC, Coulombier-Chauvel F, Link TI, Balmer D, Müller AN, Kind S, Bohnert S, Wirtz L, Chen C, Yan M, Ng V, Gautier P, Meyer MC, Voegele RT, Liu Q, Grigoriev IV, Conrath U, Brommonschenkel SH, Loehrer M, Schaffrath U, Sirven C, Scalliet G, Duplessis S, van Esse HP
Major proliferation of transposable elements shaped the genome of the soybean rust pathogen Phakopsora pachyrhizi.
Nat Commun. 2023 Apr 1;14(1):1835. doi: 10.1038/s41467-023-37551-4
Links
JGI EcoGroup Portal: Plant Pathogens
JGI PhyloGroup Portals: Fungi Dikarya Basidiomycota Pucciniomycotina Pucciniales Phakopsora