Home • Rozella allomycis CSF55 single-cell v1.0
Rozalla allomyces - single cell project
Zoospores of Rozella. (photo credit: Timothy James, University of Michigan)

The genus Rozella is mostly known to parasitize water molds, and R. allomycis is an obligate parasite of the Blastocladiomycotan fungus Allomyces. The phylum is otherwise known exclusively from a large number of environmental DNA sequences produced in aquatic, marine, and other habitats (Jones et al. 2011; Lara et al. 2011). R. allomycis grows within the host as naked protoplasm, and reproduces through the production of ephemeral zoosporangia or chitinous, thick-walled resting sporangia. The parasite requires the host to produce the cell wall of the zoosporangium, which at maturity cleave into numerous zoospores with a single flagellum. The zoospore, upon finding a suitable host, retracts its flagellum, develops a cell wall, and injects its cytoplasm into the host. The parasite protoplasm is suspected of being capable of phagocytosing the host's cytoplasm (Powell 1984). The ancestral characteristic of phagotrophy as well as phylogenetic analyses have placed the Cryptomycota at the base of the fungal tree (James et al. 2006).

Rozella allomycis has been selected as part of a Fungal single-cell genome sequencing pilot project and was sequenced using single-cell methods. A separate annotation, "Rozella allomycis CSF55", was previously sequenced using non-single-cell methods (James et al. 2013).

Genome Reference(s)

  • James TY, Pelin A, Bonen L, Ahrendt S, Sain D, Corradi N, Stajich JE
    Shared signatures of parasitism and phylogenomics unite Cryptomycota and microsporidia.
    Curr Biol. 2013 Aug 19;23(16):1548-53. doi: 10.1016/j.cub.2013.06.057. Epub 2013 Aug 8.
  • James TY, Kauff F, Schoch CL, Matheny PB, Hofstetter V, Cox CJ, Celio G, Gueidan C, Fraker E, Miadlikowska J, Lumbsch HT, Rauhut A, Reeb V, Arnold AE, Amtoft A, Stajich JE, Hosaka K, Sung GH, Johnson D, O'Rourke B, Crockett M, Binder M, Curtis JM, Slot JC, Wang Z, Wilson AW, Schüssler A, Longcore JE, O'Donnell K, Mozley-Standridge S, Porter D, Letcher PM, Powell MJ, Taylor JW, White MM, Griffith GW, Davies DR, Humber RA, Morton JB, Sugiyama J, Rossman AY, Rogers JD, Pfister DH, Hewitt D, Hansen K, Hambleton S, Shoemaker RA, Kohlmeyer J, Volkmann-Kohlmeyer B, Spotts RA, Serdani M, Crous PW, Hughes KW, Matsuura K, Langer E, Langer G, Untereiner WA, Lücking R, Büdel B, Geiser DM, Aptroot A, Diederich P, Schmitt I, Schultz M, Yahr R, Hibbett DS, Lutzoni F, McLaughlin DJ, Spatafora JW, Vilgalys R. Reconstructing the early evolution of Fungi using a six-gene phylogeny. Nature. 2006 Oct 19;443(7113):818-22. PubMed PMID: 17051209.
  • Jones MD, Forn I, Gadelha C, Egan MJ, Bass D, Massana R, Richards TA. Discovery of novel intermediate forms redefines the fungal tree of life. Nature. 2011 May 11;474(7350):200-3. doi: 10.1038/nature09984. PubMed PMID: 21562490.
  • Lara E, Moreira D, López-García P. The environmental clade LKM11 and Rozella form the deepest branching clade of fungi. Protist. 2010 Jan;161(1):116-21. doi: 10.1016/j.protis.2009.06.005. Epub 2009 Aug 11. PubMed PMID:19674933.
  • Powell, M. J. (1984). Fine structure of the unwalled thallus of Rozella polyphagi in its host Polyphagus euglenae. Mycologia 76: 1039-1048.