Info • Russula sp, TAS8 v1.0

Status

[October 2022] The Russula sp. TAS8 v1.0 genome was sequenced with PacBio, assembled with Flye, and annotated with the JGI Annotation Pipeline. The mitochondrial genome was assembled separately and is available in the downloads section.

This genome is likely a polymorphic dikaryon, and this is reflected in an assembly and annotation with significant separation of alleles. Some of the scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertoire of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.

Genome Assembly
Genome Assembly size (Mbp) 71.06
Sequencing read coverage depth 49.96x
# of contigs 776
# of scaffolds 776
# of scaffolds >= 2Kbp 776
Scaffold N50 88
Scaffold L50 (Mbp) 0.22
# of gaps 0
% of scaffold length in gaps 0.0%
Three largest Scaffolds (Mbp) 2.01, 1.14, 0.84


ESTs Data set # sequences total # mapped to genome % mapped to genome
EstClusters ESTclusters 99775 90452 90.7%
Ests est.fasta 181060175 179578526 99.2%


Gene Models FilteredModels3
length (bp) of: average median
gene 1928 1621
transcript 1570 1297
exon 264 160
intron 74 59
description:
protein length (aa) 446 342
exons per gene 5.94 5
# of gene models 13017


Funding

The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.