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Home • Saccharomyces cerevisiae M3839 v1.0
Photo of Saccharomyces cerevisiae M3839 v1.0
S. cerevisiae cells were grown in YPD medium for 16 hours, fixed, and stained with Calcofluor White, a fluorescent dye that stains the cell walls and bud scars. The scale bar represents 5 microns. Images were collected on a Zeiss LSM710 confocal microscope by Jennifer Morrell-Falvey (Oak Ridge National Laboratory).

Saccharomyces cerevisiae is the first eukaryote for which a genome was completely sequenced and it has been studied intensely as a model organism for decades (1). The physiology and genetics for this Crabtree positive, non-motile, unicellular yeast that reproduces by budding are well characterized (7). S. cerevisiae is used routinely in the baking industry and for alcohol fermentations. It is generally recognized as safe (GRAS) and tolerant to a wide range of physiological stresses such as low pH, high ethanol and high osmotic stress (2). It has gained wide-spread use as a host for recombinant protein production (5), pentose utilization capabilities have been developed (2, 4) and yeast have potential for consolidated bioprocessing (CBP) (3, 6).
The complete sequence of the robust Saccharomyces cerevisiae strain M3707, isolated from a commercial sample of distillers yeast, and its tetrads M3836-M3839 will provide a useful comparison to the previously sequenced laboratory strain S288C (SGD).




Genome Reference(s)


1.    Goffeau, A., B. G. Barrell, H. Bussey, R. W. Davis, B. Dujon, H. Feldmann, F. Galibert, J. D. Hoheisel, C. Jacq, M. Johnston, E. J. Louis, H. W. Mewes, Y. Murakami, P. Philippsen, H. Tettelin, and S. G. Oliver. 1996. Life with 6000 genes. Science 274:546-567.
2.    Hahn-Hägerdal, B., K. Karhumaa, C. Fonseca, I. Spencer-Martins, and M. Gorwa-Grauslund. 2007. Towards industrial pentose-fermenting yeast strains. Appl. Microbiol. Biotechnol. 74:937-953.
3.    Ilmén, M., R. den Haan, E. Brevnova, J. McBride, E. Wiswall, A. Froehlich, A. Koivula, S. P. Voutilainen, M. Siika-aho, D. C. la Grange, N. Thorngren, S. Ahlgren, M. Mellon, K. Deleault, V. Rajgarhia, W. H. van Zyl, and M. Penttilä. 2011. High level secretion of cellobiohydrolases by Saccharomyces cerevisiae. Biotechnol. Biofuels 4:30.
4.    Kuyper, M., M. M. P. Hartog, M. J. Toirkens, M. J. H. Almering, A. A. Winkler, J. P. van Dijken, and J. T. Pronk. 2005. Metabolic engineering of a xylose-isomerase-expressing Saccharomyces cerevisiae strain for rapid anaerobic xylose fermentation. FEMS Yeast Res. 5:399-409.
5.    Mattanovich, D., P. Branduardi, L. Dato, B. Gasser, M. Sauer, and D. Porro. 2012. Recombinant Protein Production in Yeasts. Recombinant Gene Expression, p. 329-358. In A. Lorence (ed.), vol. 824. Springer New York.
6.    Olson, D. G., J. E. McBride, A. J. Shaw, and L. R. Lynd. 2012. Recent progress in consolidated bioprocessing. Curr. Opin. Biotechnol. 23:396-405.
7.    Sherman, F. 2002. Getting started with yeast, p. 3-41. In G. Christine and R. F. Gerald (ed.), Methods Enz., vol. Volume 350. Academic Press.