Trichoderma atroviride strain B10 was produced by protoplast fusion of two Trichoderma belonging to different species, with the aim of combining multiple desirable characters in a single strain. Under standard growth conditions (PDA, 25°C and light exposure), strain B10 grows rapidly. Conidiation appears dense and radial, initially with a yellowish pigmentation that becomes a dark seaweed green over time. In biological assays on different plants (monocots and dicots) and against multiple fungal pathogens, B10 demonstrated a better performance than its parents in increasing seed germination rate, in promoting plant growth, and triggering plant defense responses to soil-borne and aerial phytopathogens.